The power of the lab in the
palm of your hand.
Meet CaDI.
The same performance as a PCR-based test but at a fraction of the cost.
The CaDI-ASAP platform is driven by chemiluminescence.
Chemiluminescence has long been recognized as the most sensitive protein detection modality but has never been implemented at the point-of-care due to the requirement of sophisticated assay timing and optimization. We have demonstrated a chemiluminescent system capable of detecting just 5 photons paired with a microfluidic device that, like a well-timed symphony, achieves all assay steps and timing in a precise fashion.
In traditional lateral flow antigen tests, reagents flow together in a linear path and are thus, inherently incompatible as they can’t achieve the required sequential delivery for an ultrasensitive chemiluminescent readout. Thus chemiluminescent tests have been limited to central labs with highly trained personnel and expensive equipment.
The innovation behind the ASAP platform is the capillary-driven immunoassay (CaDI) microfluidic device. This device is constructed from multiple layers of polyester and adhesive. These materials are used to create hydrophilic channels with integrated reagent pads. Additionally, there's a nitrocellulose strip with printed capture antibodies.
Reagents embedded in the device are released sequentially. They are delivered to the test lines in the correct order and timing. This timing can be adjusted through simple changes to the channel geometry. The device is made from common, inexpensive materials and is designed for low-cost manufacturing. This approach allows us to maintain costs significantly lower than competitors who rely on expensive PCR test cassettes.
How it Works.
All steps of a traditional ELISA are programmed onboard the cassette, including:
1. Sample delivery to the capture antibodies
2. Label antibody delivery
3. Washing of unbound material
4. Delivery of enzyme-substrate for signal generation and amplification
The role of the microfluidic network is to achieve tied sequential delivery of test reagents to the test region.
Fully automated immunoassay in a cassette. All steps of chemiluminescent ELISA (binding, washing, labeling, amplification) are performed in the microfluidic device and fully powered by capillary action with no manual intervention.
Sensitivity and specificity. Improvements of 100-1000x over traditional lateral flow tests have been demonstrated in the laboratory.
Adaptable. This system can be adapted towards any analyte with appropriate antibody pairs available by changing the reagents immobilized on the pads in the device. Reach out to learn more about our partnering opportunities. Let us bring your assay to the light!
Manufacturable. The fluidic architecture of our test cassettes are constructed with common, low-cost materials. Assembly of these devices can be readily scaled up rapidly using reel-to-reel manufacturing.
Let’s Bring Your Assay to Light!
Discover how our cutting-edge technology can be tailored to suit your unique requirements. Explore our custom solutions page to find the perfect fit for your needs.
Peer Reviewed Publications on CaDI Technology:
1) Jang I, Kang H, Song S, Dandy DS, Geiss BJ, Henry CS. Flow control in a laminate capillary-driven microfluidic device. Analyst. 2021;146: 1932–1939. doi:10.1039/D0AN02279A
2) Panraksa Y, Jang I, Carrell CS, Amin AG, Chailapakul O, Chatterjee D, Henry CS. Simple manipulation of enzyme-linked immunosorbent assay (ELISA) using an automated microfluidic interface. Anal Methods. 2022;14: 1774–1781. doi:10.1039/D2AY00326K
3) Link, J. S.; Carrell, C. S.; Jang, I.; Barstis, E. J. O.; Call, Z. D.; Bellows, R. A.; O’Donnell-Sloan, J. J.; Terry, J. S.; Anderson, L. B. R.; Panraksa, Y.; Geiss, B. J.; Dandy, D. S.; Henry, C. S. Capillary Flow-Driven Immunoassay Platform for COVID-19 Antigen Diagnostics. Analytica Chimica Acta. 2023; 1277: 341634. doi:10.1016/j.aca.2023.341634.